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Sonic hedgehog gene expression is increased in adult skeletal muscle in ovarian hormone deficient mice that wheel exercise
Author(s) -
Spence Madoka,
Childs Thomas E,
Ferreira J Andries,
Brown Marybeth
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb782
Subject(s) - skeletal muscle , endocrinology , medicine , sonic hedgehog , gastrocnemius muscle , myogenesis , gli1 , hedgehog signaling pathway , biology , chemistry , signal transduction , microbiology and biotechnology
Estrogen (E2) affects skeletal muscle pathway activation; however, the mechanisms by which E2 regulates skeletal muscle are not yet fully known. There is increasing evidence that (1) Sonic hedgehog (Shh) pathway is involved in myogenesis and hypertrophy, and (2) estrogenic compounds affect Shh pathway in vivo. The purpose of this study was to examine the relationship between E2, physical activity, and Shh pathway in adult skeletal muscle. In this study, we tested the hypothesis that both E2 and physical activity would increase Shh pathway gene expression. Female C57/BL6 mice (4 mos) were divided into 4 groups: Intact control, Intact run, Ovariectomy (Ovx) control, and Ovx run. Mice were on running wheels for 6 weeks. Ovx was done prior to wheel running. Shh pathway activation was measured in gastrocnemius muscle by mRNA expression of Shh signaling proteins, Gli1 and Ptch1. Gli1and Ptch1 mRNA expression was significantly increased in the Ovx run group compared to Ovx control, however exercise did not affect the intact mice. Ovx mice ran 38% the distance of Intact run. Muscle size was unaffected by running in both groups. However, muscle force/area (Po/mass) in Ovx run was significantly less than Intact run (p<0.05). Our results indicate that Shh pathway is upregulated in adult skeletal muscle when E2‐deficient animals undergo voluntary exercise. Support: MU Research Board Grant