Premium
Reduced ventilatory function and sarcopenia of the diaphragm muscle in a mouse model of advanced aging
Author(s) -
Greising Sarah M.,
Sieck Dylan C.,
Mantilla Carlos B.,
Sieck Gary C.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb779
Subject(s) - sarcopenia , ventilation (architecture) , diaphragm (acoustics) , diaphragm muscle , medicine , respiratory minute volume , respiratory system , tidal volume , senescence , endocrinology , cardiology , mechanical engineering , physics , acoustics , loudspeaker , engineering
Aging is associated with respiratory complications and muscle dysfunction that may reduce the ability to perform expulsive, non‐ventilatory behaviors. We hypothesized that ventilatory function is impaired with aging and that the diaphragm muscle (DIAm) is susceptible to sarcopenia. Unrestrained‐awake ventilatory function, DIAm specific force, and fiber cross‐sectional area (CSA) were examined in a validated mouse model of advanced aging (expression of a hypomorphic allele ( H ) of BubR1 ), naturally aged (28 mo) and adult (5 mo) wild type ( wt ) mice (same genetic background). Minute ventilation and breathing frequency were decreased 17% in BubR1 H/H and 33% in aged wt mice vs. adult wt mice. Tidal volume was not different across groups. DIAm specific force was lower in both aged mice groups compared to the adult wt mice, being 62% lower in BubR1 H/H mice. DIAm fiber CSA from BubR1 H/H and aged wt mice was smaller than adult wt mice (23 and 10%, respectively). These results indicate that aging is associated with reduced ventilatory function and evidence of DIAm sarcopenia. In addition, aged mice recruit a greater percentage of DIAm fibers for eupneic breathing (greater fraction of maximum force). Together, these age‐related changes may limit the ability to accomplish expulsive, non‐ventilatory behaviors essential for airway clearance, and thus contribute to respiratory complications. Supported by Mayo Clinic.