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Role of Probiotic Bifidobacterium Infantis after Oral Feeding in TNBS Rat Model of Colitis
Author(s) -
Javed Najma Hassan,
Furman David
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb771
Subject(s) - colitis , probiotic , bifidobacterium , medicine , inflammatory bowel disease , inflammation , feces , gastroenterology , food science , biology , disease , microbiology and biotechnology , lactobacillus , bacteria , genetics , fermentation
Role of dietary factors in disease prevention has recently gained more attention. Bifidobacterium Infantis (BI) are non‐pathogenic living organisms implicated with therapeutic benefits in GI inflammatory disorders. Little is known about the regulatory mechanism of individual probiotics on intestinal function. We studied effects of feeding BI in TNBS rat model of Colitis. Twelve rats were divided into 2 groups. One received oral gavage of 0.205 g of BabyLife B. Infantis in distilled water. Sham‐fed rats were fed veh for 7 days. Colitis was induced and feeding continued for 7 days post induction. Animals were monitored for weight loss, water & food intake, blood loss, stool activity and ranked following a Disease Activity Index. Rats were euthanized and tissues were harvested & examined blindly. Tissues were set up in Ussing Chambers for functional studies. Feeding of BI significantly decreased the inflammation (p=0.007) indicated by decrease ulceration, intestinal and peritoneal adhesions, wall thickness and presence of Peyers patches. Electrical field stimulation indicated a trend towards higher responses in BI fed tissues as compared to sham‐fed colitis tissues. Our study indicates that feeding Bifidobacterium Infantis beneficially modifies the inflammatory responses in rat model of colitis. Study indicates preventive and therapeutic value of probiotics.