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Regulation of ZnT1, Zip6 and MT‐1 mRNA in cultured choroid plexus elicited by manipulation of extracellular zinc (Zn)
Author(s) -
Aquino Mayra,
Young Robin K,
Villalobos Alice R
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb758
Subject(s) - choroid plexus , metallothionein , messenger rna , extracellular , intracellular , zinc , downregulation and upregulation , transporter , chemistry , gene expression , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry , gene , central nervous system , organic chemistry
Regulation of Zn transport in choroid plexus as dietary Zn changes remains elusive. We seek to elucidate the modulation of Zn exporters (ZnTs), Zn importers (ZIPs) and the Zn‐binding protein, metallothionein (MT) in choroid plexus induced as Zn availability changes. To begin, we used real‐time qRT‐PCR to analyze ZnT1, ZIP6 and MT‐1 gene expression in Zn‐deprived and Zn‐supplemented cultured choroid plexus epithelial cells. Cells were treated for 12h‐48h with 10 μM DTPA, an extracellular Zn chelator, or 25 μM ZnCl 2 . At 12h‐48h, DPTA decreased ZnT1 mRNA by 60% and increased Zip6 mRNA by 75%. MT‐1 mRNA decreased 65% at 12h‐24h and further decreased at 48h. Zn supplementation increased ZnT1 mRNA by 75% at 12h and by 250% at 18h‐48h. Zip6 and MT‐1 mRNA increased 6‐fold and 110‐fold at 12h; Zip6 and MT‐1 mRNA then decreased incrementally, but were 4‐fold and 10‐fold greater than Control at 48h. Although these preliminary data do not reflect integrated changes in the full complement of Zn transporters elicited as Zn intake changes, they suggest coordinated modulation of Zn transporters, e.g., ZnT1 and Zip6, and MT, might regulate Zn transport and intracellular Zn accumulation and availability in choroid plexus.