Premium
Characterization of KISS‐1 Expression and Cell Viability in MCF7 Breast Cancer Cells After Exposure to Gamma‐Hexachlorocyclohexane
Author(s) -
Joseph Shanelle,
D'Auvergne Oswald
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb71
Subject(s) - viability assay , metastasis , cancer research , biology , breast cancer , cancer , cell , cancer cell , cell growth , andrology , chemistry , medicine , genetics
While it is known that exposure to organochlorines may cause epigenetic birth defects in the offspring of individuals exposed to such chemicals, the role of organochlorines in cancer progression is unknown. KISS‐1 is presumed to be a metastasis suppressor gene and large amounts of its associated protein, kisspeptin‐54, has been associated with aggressive and fatal cases of breast cancer without further elucidation. In an effort to define the role of organochlorines in breast cancer progression we have exposed MCF7 breast cancer cells to a 0.2 millimolar solution of gamma‐hexachlorocyclohexane at 2.5 and 5 microliters per millimeter for a period of twenty‐four hours. MTT cell proliferation assays revealed that cell viability decreased as the concentration of chemical exposure increased. p53 was expressed in all samples during pcr analyses while expression of cyclin‐A2 struggled to persist. There was a significant loss of cytoplasm and decreased nuclear visualization in the treated cells as compared to the control which suggests the chemical promotes death in the cancer cell line. KISS‐1 which was expressed in all parts of the MCF7 cells whether treated or control showed significant nuclear translocation and a diminutive increase in concentration in small populations of cells exposed to gamma‐HCH. This data suggests that exposure to gamma‐HCH does not contribute to aggressive behavior nor metastasis in MCF7 cells.