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Central effects evoked by proline‐rich decapeptide in rats: changes in cardiovascular parameters and neuronal c‐Fos
Author(s) -
Ianzer Danielle Alves,
Xavier Carlos Henrique,
Madden Christopher J.,
Camargo Antonio C.,
Morrison Shaun F.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb696
Subject(s) - c fos , chemistry , bradycardia , mean arterial pressure , nitric oxide , medicine , endocrinology , saline , blood pressure , heart rate , medulla oblongata , pharmacology , central nervous system , biochemistry , gene expression , gene
Bj ‐PRO‐10c (10c) is a proline‐rich oligopeptide from Bothrops jararaca CNP brain precursor and crude venom. 10c evokes antihypertensive and bradycardic effects in SHR. In addition, 10c crosses the blood brain barrier and increases plasma levels of the nitric oxide precursor, L‐arginine. We evaluated whether centrally injected 10c changes blood pressure, heart rate (HR) and the expression of neuronal c‐Fos . 10c (7.1nmol/Kg) or vehicle (NaCl 0.9%) were icv injected in anesthetized male SHR and Wistar rats. Mean arterial pressure (MAP) and HR were recorded for 90min following injection. Brain sections were taken to evaluate c‐Fos and NOS, by double‐labeling immunohistochemistry. Although 10c reduced MAP and HR in both Wistar (ΔMAP: −2±1 saline vs . −6±1mmHg 10c, ΔHR −13±5 vs . −34±5bpm; p <0.01) and SHR (ΔMAP −7±2 saline vs . −16±3mmHg 10c, ΔHR −13±5 vs . −42±7bpm; p <0.01), the changes in MAP were greater ( p <0.01) in SHR. At 90min following 10c injection, c‐Fos expression was increased in comparison to saline injections in the nucleus Ambiguus and CVL, areas, which contained neurons double‐labeled for c‐Fos + NOS. We conclude that the central administration of 10c prominently drives a bradycardia, possibly through NO‐related mechanisms. Support: Fapesp