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Expression of Anti‐angiogenesis protein, Tenomodulin, in Vascular Smooth Muscle Cells
Author(s) -
Xing Enze,
Wen Yuan,
Franz Michael G
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb674
Subject(s) - angiogenesis , biology , pathology , microbiology and biotechnology , medicine , cancer research
Tenomodulin (Tnmd) was expressed predominantly in hypovascular connective tissues with anti‐angiogenesis function. Interaction between endothelial cells and vascular smooth muscle cells (VSMCs) plays important role in maintaining normal vascular structure and function, such as angiogenesis. This study investigated Tnmd expression in rat VSMCs (rVSMCs) and explored regulation of Tnmd expression in response to different stimulations. rVSMCs were isolated from aorta of SD rats and maintained under defined in vitro conditions. Tnmd protein and mRNA were detected by immunohistochemistry (IHC), immunofluorescence staining (IF), Western blot, RT‐PCR, and DNA sequencing, respectively. Cells were treated with proinflammatory cytokines, growth factors or immune modulator to induce changes in Tnmd expression. rVSMCs express Tnmd both at mRNA and protein level. ICH and IF showed that Tnmd was located in smooth cell layer of media artery and rVSMCs. Western blot showed Tnmd specific band of rVSMCs located at the same position as that of fibroblast. DNA sequencing on targeted RT‐PCR product from rVSMCs showed 100% identity with that from rat linea alba fibroblast. Tnmd protein level significantly decreased in rVSMCs after 72‐h treatment of PDGF till 120 h. Real‐time RT‐PCR showed that PDGF treatment decreased Tnmd mRNA expression. Data suggest change of Tnmd expression in VSMCs is involved in local regulation of angiogenesis in certain physiological and pathophysiological conditions. This study was supported by Department of General Surgery, University of Michigan medical School.