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Temporal gene expression profiling of the hypothalamus following trauma
Author(s) -
Bowman Phillip Dalton,
Bynum James A.,
Craig Teresa,
Meledeo M. Adam,
Darlington Daniel N.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb673
Subject(s) - polytrauma , transcriptome , glucocorticoid receptor , hypothalamus , microarray , fold change , gene expression , biology , wnt signaling pathway , gene expression profiling , medicine , receptor , endocrinology , gene , microbiology and biotechnology , signal transduction , surgery , genetics
The hypothalamus plays an important role in homeostatic regulation. In order to identify biological processes relevant to the response to severe traumatic injury, the hypothalamic transcriptome was examined following polytrauma. Sprague‐Dawley rats (300–400g) were anesthetized with isoflurane and trauma induced by damaging the small intestine, left and medial liver lobes, right leg skeletal muscle, and a right femur break. Rats had 40% of their blood volume removed in 30–60 min with no resuscitation. All rats survived the 240 min experimental period. Brains were removed at 0.5, 1, 2, 3, and 4 hours post injury and placed in RNAlater.™ Hypothalami were dissected from 3 mm coronal sections (4 per time point) and RNA extracted. Following labeling and hybridization to rat whole genome microarrays (Agilent), genes statistically altered in their expression compared to sham were determined with BRB Arraytools. Within 30 min, 350 genes were upregulated >2‐fold and 660 were downregulated >2. By reference to Ingenuity Pathways Analysis program, canonical pathway activation included genes reported to be involved in hepatic stellate cell activation as well as glucocorticoid receptor, Il–10, acute phase response, and G‐protein coupled receptor signaling. Understanding the hypothalamic response to polytrauma may provide new targets for improving survival. Project funding provided by US Army Medical Research and Material Command