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Ethyl pyruvate protects against myocardial ischemia/reperfusion injury via mitochondrial membrane potential dependent and anti‐apoptotic mechanisms
Author(s) -
Chun Byeong Jo,
Moon Jeong Mi,
Lee Byeong Guk
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb669
Subject(s) - apoptosis , reperfusion injury , messenger rna , flow cytometry , ischemia , blot , chemistry , mitochondrion , microbiology and biotechnology , cardioprotection , andrology , biology , medicine , biochemistry , gene
Purpose A role Ethyl pyruvate (EP) in ischemia/reperfusion (I/R) injury is not clear. We investigated whether EP could inhibit cardiac myocyte apoptosis and reduce mitochondrial injury in the ischemic/reperfused heart of the mice. Methods Male C57BL/6 mice of 12–15 weeks of age were subjected to 25 min of ischemia followed by 45 min of reperfusion. A modified KH perfusion fluid was used, contained except where EP levels were varied. JC‐1 staining flow cytometry was used to examine mitochondrial membrane potential. We investigated mitochondrial structure, apoptosis rate and Bcl‐2, Bax, Caspase‐3 proteins. RT‐PCR was used to determine the expression of P38MAPK mRNA and JNK mRNA. Western blotting was used to measure the expression of P38MAPK, JNK and NFκB P65. Results Our results show that 4.0 mM EP group increased LVDP recovery rate and decreased mitochondrial ultrastructural damage as compared to other EP groups. The apoptosis rate and PCI of Bax, Caspase‐3 proteins in the 4.0 mM EP group were decreased, while the PCI of Bcl‐2 protein was increased. The expression of P38MAPK mRNA, JNK mRNA and protein of P38MAPK, JNK and NFκB P65 in free EP group was increased. The rate of loss Δψm cells in the 4.0 mM EP group was lower than in the other groups. Conclusion Our dates suggest that the EP plays an important role in protection of the myocardium against I/R injury and may represent a novel therapeutic target.