Premium
Intratracheal instillation of TiO 2 increases carotid‐femoral pulse wave velocity by suppression of eNOS and increasing IL‐6 in artery in rat
Author(s) -
Wang Jiun-Jr,
Wang Chin-Hao
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb602
Subject(s) - malondialdehyde , pulse wave velocity , enos , femoral artery , creatine kinase , medicine , nitric oxide , blood pressure , cardiology , chemistry , endocrinology , nitric oxide synthase , oxidative stress
Background In addition to the damages to the lungs, inhaled nanoparticles may induce systemic proinflammatory and prothrombotic effects; however, its impacts to the alternation of arterial wall property have not been investigated. Methods Studies were performed on 32 male Sprague‐Dawley rats in 4 groups, in terms of different interventions [intratracheal instillation of titian dioxide (TiO 2 ; D=20 nm; 4 mg/kg) or vehicle] and treatments [N‐acetylcysteine (NAC; 20 mg/kg; i.v.) or Xigris (10 mg/kg; i.v.); 5 min prior to TiO 2 instillation]. The LV function and the carotid‐femoral pulse wave velocity (CFPWV) were assessed by the LV pressure‐volume and two intravascular pressure transducers, respectively. Blood were samples at baseline, 1, 3 and 5 hrs after TiO 2 to assess serum creatine phosphokinase (CPK) and creatine kinase‐MB (CK‐MB). Nitric oxide (NO), methylguanidiene (MG), malondialdehyde (MDA) and C‐reactive protein (CRP) were examined at 5 hrs. The expressions of IL‐6 and eNOS in the thoracic aorta were examined. Results TiO 2 instillation induced severe systemic thrombotic effects, increasing CFPWV by 50% (8.8±3.6 m/s vs. 13.7±4.0 m/s). Xigris pretreatment attenuated thrombotic effects with minor changes in biomarkers and CFPWV (12.9±4.1 m/s). NAC pretreatment attenuated the increase in CFPWV (10.1±2.6 m/s; P<0.05) along with reduced serum MG, MDA and CRP and tissue IL‐6 and increased eNOS, while thrombotic effects persisted. Conclusion Intratracheal instillation of TiO 2 induced transit increase in arterial stiffness is associated with systemic inflammatory responses.