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IMP2 Expression In The Mouse Nervous System
Author(s) -
Chmielewski Sarah Elizabeth,
Black Paige E.,
Holmes Tiffany,
Lau Kelly,
Bassell Gary J.,
Xu Mei
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb60
Subject(s) - immunocytochemistry , gene isoform , nervous system , microbiology and biotechnology , messenger rna , axon , translation (biology) , western blot , biology , gene expression , regeneration (biology) , central nervous system , northern blot , antibody , neuroscience , gene , immunology , genetics , endocrinology
Insulin ‐ like growth factor‐II (IGF‐II) mRNA‐binding protein‐2 (IMP2) is one of the three homologs (IMP1–3) that play important roles in the posttranscriptional regulation of gene expression in several tissues. IMP1/ZBP1 (zipcode binding protein) has been shown to play important roles in axon guidance and regeneration by regulating the localization and translation of specific mRNAs. However, the function of IMP2 is least understood, largely because an isoform‐specific antibody is not available, which makes the conventional techniques to locate protein expression not feasible. We custom made an IMP2‐specific antibody. We used Western blot and immunocytochemistry to test its specificity on cultured cells following over expression of IMP 1–3 isoforms, respectively. Using this IMP2‐specific antibody, we examined IMP2 expression in the mouse nervous system. We found that IMP2 expression in the nervous system is sustained postnatally, unlike that of IMP1 and IMP3. Ongoing experiments are aimed at further understanding IMP2 expression patterns during injury and assessment of its role to facilitate mRNA localization during axon regeneration in the adult nervous system. This work is supported by the Department of Anatomy, PCOM and Center for Chronic Disorders of Aging (CCDA).