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Analysis of Lindane and Metabolites by HPLC‐UV‐Vis and MALDI‐TOF
Author(s) -
Naidu Naga V,
Smith-Baker Charlotte,
Yakubu Momoh A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb590
Subject(s) - lindane , chemistry , urine , high performance liquid chromatography , chromatography , metabolite , hexachlorocyclohexane , pesticide , biology , biochemistry , agronomy
Lindane (hexachlorocyclohexane) is a persistent environmental toxicant and a known neurotoxicant. High levels of Lindane have been detected in postmortem human brain from Parkinson disease patients. Metabolism of Lindane produces several metabolites which are still imperfectly identified. Therefore, easy, fast and sensitive method to detect Lindane is highly desirable. We determined Lindane and its metabolites in urine by HPLC‐UV‐Vis and characterized it with MALDI‐TOF. Rats received Lindane (17.6 mg/Kg) or vehicle orally for 4 wk and urine collected for analysis. Lindane and metabolites were extracted and analyzed by HPLC followed by MALDI‐TOF characterization. HPLC spectrum of standard Lindane peaked at 6.0 min. and urine samples (n=6) peaked at 6.10–6.30 min. Control urine did not show any peak corresponding to Lindane (6.0–6.30 min). MALDI‐TOF analysis of standard Lindane showed a peak corresponding to 293 m/z whereas the urine samples showed major peaks at 292–293 m/z corresponding to Lindane along with minor peaks at 259, 269, 277, and 318 m/z corresponding to Lindane metabolites: ‐ chlorobenzene, ‐chlorophenol, ‐hexacholorohexane, and other derivatives. These results show that HPLC is sensitive for detecting Lindane and its metabolites and MALDI‐TOF can identify specific metabolites of Lindane in urine by matching spectrum peaks generated to the molecular weights of the metabolites. Thus, HPLC‐UV‐Vis‐MALDI‐TOF analysis can be a reliable, non‐invasive method of detecting lindane exposure and burden. Supported by NHLBI (HL‐70669 and HL03674)