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Scopoletin induces melanogenesis through CREB/PKA and p38 MAPK signaling pathway
Author(s) -
Mun Yeun-Ja,
Kim Dae-Sung,
Lee Young-Eun,
Woo Won-Hong
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb55
Subject(s) - scopoletin , p38 mitogen activated protein kinases , creb , phosphorylation , mapk/erk pathway , chemistry , melanin , protein kinase a , microphthalmia associated transcription factor , microbiology and biotechnology , biochemistry , biology , tyrosinase , medicine , transcription factor , enzyme , gene , alternative medicine , pathology
In this study, we investigated the molecular events of melanogenesis induced by scopoletin in B16 melanoma cells. To evaluate melanin relative expression, using RT‐PCR and western blotting, B16 cells were incubated for 24 h with scopoletin. Scopoletin induced the expression of tyrosinase, TRP1 and MITF proteins. Moreover, it increased the phosphorylation ERK, CREB and p38 MAPK. Scopoletin‐induced melanogenesis was reduced by H‐89, KT5720, PKA inhibitors, or SB203580, a p38 MAPK inhibitor, but not by Ro 320432, a PKC inhibitor, which suggests the involvement of PKA and p38 MAPK in scopoletin induced melanogenesis. Scopoletin induced the activation of CRE, which suggests that scopoletin‐induced melanogenesis is mediated by PKA, which occurs downstream of scopoletin‐increased cAMP production. This result was further confirmed by the fact that scopoletin‐induced phosphorylation of CREB was inhibited by H‐89, but not by PD98059, a MEK1 inhibitor. These results demonstrate that scopoletin induces melanogenesis through CREB/PKA and p38 MAPK signaling pathway.