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Complete artificial saliva alters expression of proinflammatory cytokines in human dermal fibroblasts
Author(s) -
MALPASS GLORIA,
PRASAD GADDAMANUGU,
HOWLETT ALLYN
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb549
Subject(s) - proinflammatory cytokine , interleukin 8 , saliva , interleukin , chemistry , immunology , microbiology and biotechnology , biology , cytokine , inflammation , biochemistry
Complete artificial saliva (CAS) is a saliva substitute often used as a solvent control/vehicle for test reagents. In cultured normal human dermal fibroblasts incubated for 5 hours with CAS, gene expression for proinflammatory cytokines including interleukin 8 (IL8) and interleukin 1á (IL1á) was increased. Expression of vascular cell adhesion molecule 1 (VCAM1), a gene upregulated by certain proinflammatory cytokines including IL1á, was also increased. Furthermore, cytometric bead array assays indicate that CAS increased the release of proinflammatory cytokines including IL8 into the cell culture media. These results suggest that constituents of CAS may induce a proinflammatory response in cultured human dermal fibroblasts. Therefore, it is important to carefully evaluate/consider the “vehicle effects” of CAS and its constituents in oral/dental research. Funded by RJ Reynolds Golberg Postdoctoral Fellowship to GEM.