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Anti‐inflammatory effects of Laminaria japonica in association with the inhibition of nitric oxide and pro‐inflammatory cytokines production
Author(s) -
Kim Young Woo,
Cho Je,
Kim Sang Chan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb547
Subject(s) - chemistry , carrageenan , nitric oxide , lipopolysaccharide , proinflammatory cytokine , pharmacology , traditional medicine , inflammation , biochemistry , immunology , medicine , organic chemistry
Laminaria japonica has been frequently used as a drug in traditional oriental medicine, and as food supplements in many of the Asian countries. This research investigated the effects of Laminaria japonica extract (LJE) on the paw edema formation in rats and on the induction of nitric oxide (NO), prostaglandin E2 (PGE2) and proinflammatory cytokines by lipopolysaccharide (LPS) in Raw264.7 cells. The effect of LJE on acute phase inflammation was evaluated in a carrageenan‐induced paw edema model, as assessed by histomorphometric and immunohistochemical analyses. NO, PGE2, TNF‐α, interleukin‐1β and interleukin‐6 contents were assayed by ELISA. iNOS and COX‐2 expressions were determined by western blot analyses. In rats, LJE treatment inhibited carrageenan‐induced paw edema formation, and infiltration of inflammatory cells in H&E staining. LJE treatment prevented the ability of LPS to increase the levels of iNOS and COX‐2 protein in a concentration‐dependent manner. Consistently, LJE suppressed the production of TNF‐α, interleukin‐1β and interleukin‐6. Treatment of the cells with LJE caused inhibition of I‐κBα phosphorylation induced by LPS suggesting LJE repression of NF‐κB activity by LPS. In conclusion, this study showing here may be of help to understand the pharmacology and action mechanism of LJE, and the anti‐inflammatory use of Laminaria japonica.