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Decreased release of methyl palmitate in perivascular adipose tissue in hypertension
Author(s) -
Chang Hsi-Hsien,
Lee Yuan-Chieh,
Chiang Chih-Lung,
Liu Chin-Hung,
Chen Mei-Fang,
Chen Po-Yi,
Lee Tony J. F.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb532
Subject(s) - losartan , chemistry , endocrinology , medicine , adipose tissue , pharmacology , angiotensin ii , blood pressure , biochemistry
Our recent studies have demonstrated that palmitic acid methyl ester (PAME) is the major perivascular adipose tissue (PVAT)‐derived relaxing factor. We determined if PAME release from the PVAT and/or its vasorelaxing activity decreased in hypertension. PAME release from aortic PVAT preparations of Wistar Kyoto rats (WKY) and spontaneously hypertensive rat (SHR, 20‐week old) was estimated pharmacologically using superfusion bioassay cascade technique and biochemically by GC/MS. Results indicated that the PVAT of WKY and SHR aorta spontaneously released PAME. The release was significantly less but with an increased release of angiotensin II in SHR. Also, PAME‐induced relaxation of SHR aortic smooth muscle diminished drastically, which was reversed by losartan. Incubation of isolated PVAT preparations of SHR in Krebs solution containing losartan (10 £gM) for 30 min enhanced PVAT‐elicited vasorelaxation, with enhanced PAME release. These results suggest a noble role of PVAT & PAME in pathogenesis of hypertension. Antihypertensive effect of losartan is partly attributed to its increasing PAME release and reversing diminished PAME‐induced vasorelaxation (Supported by NSC, Tzu Chi Found & TCU).