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Induction of apoptosis in HepG2 cells by methanol extract of Ulmus pumila
Author(s) -
Ghosh Chiranjit,
Hwang Seong-Gu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb517
Subject(s) - apoptosis , ulmus pumila , chemistry , traditional medicine , inflammation , pharmacology , biology , microbiology and biotechnology , immunology , biochemistry , medicine , botany
Ulmus pumila. L is commonly used as a traditional Chinese medicine for treating edema, mastitis, gastric cancer and inflammation. In this study, we examined apoptosis of HepG2 cells induced by methanol extract of Ulmus pumila (UP), and the mechanism underlying apoptosis was also investigated. UP inhibited proliferation of HepG2 cells in dose and time dependent manner. Treatment of HepG2 cells with UP (50– 400 mg/ml) for 24 and 48 h led to a significant dose‐dependent increase in the percentage of cells in sub‐G1 phase by analysis of the content of DNA in cells. Immunoblot analysis showed that UP stimulated the apoptotic factors bcl‐xl, caspase‐3 and PARP in HepG2 cells. The results indicate that UP induces programmed cell death (apoptosis) in HepG2 cells and can be a potential therapeutic agent for treatment of hepatocellular carcinoma.