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Morphometric analysis of the intestinal villi in experimental administration os semicarbazide
Author(s) -
Cabeças José Ricardo,
Marques Pedro,
Farinha Rodrigo,
Silva Fernando Capela,
Cabrita António Silvério
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb504
Subject(s) - small intestine , gastroenterology , anatomy , chemistry , biology , medicine , pathology
The objective of this study was to evaluate the morphologic changes of the small intestine related to lathyrism. Thirty animals were randomly distributed by three groups of ten animals each. The control group (CP), had no manipulation. In the groups 2 and 3 we incorporated semicarbazide in the diet, in the doses of 3 and 6mg/kg, respectively, during 4 weeks. By the 30th day, all animals were killed with an overdose of anesthetics and the complete necropsy was performed. Histopathological processing and analysis of the small intestine was made. Pictures of routine stained slides were taken and the length of the small intestine villi was measured using the software ImageJ. The intestinal villi were distributed over 5 classes (very small‐VS, small‐S, medium‐M, big‐B and very big‐VB) using the average and standard deviation, for the CP. In the CP the percentage of each class was: 2 VS; 16 S; 64 M; 16 B; 2 VB. In the group 2: 87 VS; 13 S; 6 M; 1 B; 0 VB. In the group 3: 73 VS; 24 S; 3 M; 0 B; 0 VB. Significant changes were found, with the increase of the number of small villis in groups 2 and 3. These results suggest that an adequate collagen synthesis is necessary to maintain the intestinal villi length.