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The prostate in DMBA administration – Morphometric evaluation
Author(s) -
Barandas Gustavo Teixeira,
Pereira Filipe,
Costa Eduardo,
Farinha Rodrigo,
Cabrita Silverio
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb503
Subject(s) - dmba , prostate cancer , prostate , carcinogen , chemistry , pathology , physiology , medicine , pharmacology , biochemistry , cancer , carcinogenesis
7,12‐Dimethylbenz(a)antracene (DMBA) is a xenobiotic found in cigarette smoke and exhaust gases of combustion engines. It is an aromatic polycyclic hydrocarbon, metabolized by P450 enzymes producing reactive metabolites which form covalent adducts with DNA and the formation within DNA of depurinated abasic sites. The aim of this study is the evaluation of DMBA action on prostate by morphometric and histopathological analysis. Ten Wistar male rats, 8 weeks old, were submitted to DMBA administration, 20 mg/Kg/day, three times a week. The control group was kept without manipulation. All the animals were killed after two weeks, and a complete necropsy was performed. The prostate fragments were stained by H&E and the pieces lined by epithelia were measured using the software Image J. The frequencies distribution of the control group were achieved and considered as a reference value, for test group comparison. No statistic significance was found. The histology evaluation of the prostate of test group show no morphologic lesions. These results allow us to suggest that the acute intoxication with DMBA, as in this model, has no morphologic translation.