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Mast cell role in pulmonary hypertension in rat models
Author(s) -
Lawrenz Josh M,
Baybutt Richard,
Baidoo Cletus,
Dim Daniel,
Chang Quin,
Herndon Betty,
Poisner Alan,
Molteni Agostino
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb493
Subject(s) - adventitia , medicine , vasculitis , fibrosis , bleomycin , mast cell , lung , pathology , degranulation , lumen (anatomy) , immunology , receptor , chemotherapy , disease
Elevated numbers of mast cells were found in a rat model of fat embolism and pulmonary vasculitis and fibrosis where the vasculitis was related to increased secretion of angiotensin II. Our early work suggests that this increase could be consequent to an increase in renin secretion produced by an increased number of mast cells. This study reports mast cell response in another lung damage model also associated with severe vasculitis and fibrosis: Bleomycin administration. Twenty four rats were divided between diets receiving 15% flax seed oil or corn oil controls. The groups were further divided to receive intratracheally 8 units/kg of bleomycin or vehicle. After 7 days, rats were euthanized and lungs harvested for histological studies. Arteries were measured for lumen patency and the ratio of media/adventitia diameter. Mast cells were counted in Evans Blue stained sections. Bleomycin induced marked reduction of vascular lumen patency and media/adventitia ratio (p< 0.01), similar to that obtained with fat embolism. Mast cell number, however, was not increased. Flax seed oil treatment markedly reduced vasculitis and fibrosis without influencing mast cell number and location (mostly around peribronchial arteries and subpleural septa). Contrarily to what was observed in the fat embolism model mast cells did not correlate with vasculitis and fibrosis, suggesting a different pathogenic mechanism. Wheaton College