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Neutrophil recruitment in experimental sepsis: hypertonic solution action
Author(s) -
Theobaldo Mariana,
Llimona Flavia,
Soriano Francisco Garcia
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb485
Subject(s) - sepsis , myeloperoxidase , tonicity , saline , medicine , basal (medicine) , lung , pharmacology , immunology , chemistry , gastroenterology , inflammation , insulin
Sepsis is a systemic inflammatory response to infection and a major cause of mortality. Hypertonic saline solution (HSS) showed improvement on hemodynamic and inflammatory response. We studied the HSS effects in sepsis through cecal ligation and puncture (CLP) in mice Balb‐C, divided in 4 groups: CLP without treatment (CLP); CLP treated with saline solution 0,9% ‐ 34ml/Kg (SS); CLP treated with HSS 7,5% ‐ 4ml/Kg (SH); and a basal group without CLP and treatment; both treatments were applied 30 min after CLP. Lung and gut were collected after 6, 12 and 24h to analyze neutrophil recruitment using myeloperoxidase as index (MPO), ICAM, VCAM by ELISA and differential cell counter from peritoneal fluid. The SH group showed higher survival rate (60%) than SS (46,6%) and CLP (33,3%). CLP group (3 ± 0,4mU/mg; 29,4 ± 5,8 ng/mg) increased MPO and ICAM levels when compared to SH (1,5 ± 0,2 mU/mg; 15,9 ± 2,3 ng/mg) in lung at 24h. In gut MPO level increased in SH (0,9 ± 0,3mU/mg) when compared to CLP (0,1± 0,03mU/mg) at 6h; at 12h and 24h, SH decreased (0,2 ± 0,08; 0,2±0,05 mU/mg) in relation to CLP (3,5 ± 1,4; 0,6 ±0,05 mU/mg), ICAM levels in SS (1,7 ± 0,1 ng/mg) increased compared to SH (1,13±0,06ng/mg) at 6h, and SH (0,9±0,07ng/mg) decreased in relation to CLP (1,3 ± 0,07ng/mg) and SS (1,3 ± 0,1 ng/mg) at 12h. HSS protects the lung against tissue damage during sepsis, decreasing neutrophil recruitment into tissue through of a reduction in ICAM. CAPES.