Premium
Exogenous Calcium Induces Autophagy via Classical Autophagic Machinery and Recruits Endoplasmic Reticulum Membranes to Form Autophagosomes
Author(s) -
Chen Xi,
Li Min,
Gao Wentao,
Yin Xiao-Ming
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb478
Subject(s) - autophagy , endoplasmic reticulum , microbiology and biotechnology , autophagosome , chemistry , atg5 , pi3k/akt/mtor pathway , golgi apparatus , calcium , unfolded protein response , membrane , biochemistry , biology , signal transduction , apoptosis , organic chemistry
Calcium plays a role in regulation of autophagy. We have reported that exogenously added calcium as the calcium phosphate precipitate (CPP) induces autophagy. Here we further show that CPP‐induced autophagy applies classical autophagic machinery including LC3 conjugation molecules Atg4, Atg5 and Atg3, and autophagosome nucleation molecules FIP200 and Beclin1; however, Atg9 is not involved. CPP does not induce mTOR inhibition, neither over‐production of radical oxygen species, and only slightly increases endoplasmic reticulum (ER) stress. CPP activates DFCP1 in a PI3k and calcium dependent manner, and increases the association of DFCP1 with autophagosomes. ER membrane, but not Golgi and mitochondria membranes, comprises part of the LC3 positive autophagsomes. These data suggests CPP works through non‐mTOR dependent mechanisms to activate downstream classical autophagosome nucleation and expansion molecules, and the growth of autophagosomes induced by CPP is closely related to ER.