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EGCG Exerts Antioxidant Protection in Unilateral Testicular Torsion
Author(s) -
Al-Maghrebi May,
Renno Waleed,
Al-Ajmi Nada
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb462
Subject(s) - testicular torsion , oxidative stress , nadph oxidase , medicine , spermatogenesis , endocrinology , antioxidant , superoxide dismutase , andrology , chemistry , atrophy , biochemistry , surgery
Objective This study aimed at examining the protective role of EGCG on the enhanced oxidative stress status during testicular ischemia reperfusion injury (IRI). The influence of EGCG on the testicular antioxidant system and loss of ipsilateral testicular spermatogenesis was also studied. Materials and methods Eighteen SD male rats (250–300g) were randomly assigned into three groups. Group I was the sham rats, Group II rats underwent unilateral torsion‐detorsion (UT‐D) only while group III rats underwent UT‐D with EGCG pretreatment. Rats underwent 1 hour torsion (ischemia) and 4 hours detorsion (reperfusion). Damage to spermatogenesis was assessed by H&E staining and Johnson scoring. Oxidative stress was detected by measuring SOD activity, MDA concentrations and mRNA expression of NADPH subunits p22 phox , p47 phox and gp91 phox . Results UT‐D to the left ipsilateral testes resulted in a noticeable seminiferous tubular atrophy, reduction of the number of germ cell layers and arrest of spermatogenesis. Testicular SOD activity was significantly reduced during UT‐D (p< 0.05) with a parallel increase in MDA concentrations (p<0.05) possibly caused by the accumulation of H 2 O 2 . A significant increase in the mRNA expression of NADPH oxidase subunits p22 phox , p47 phox and gp91 phox during UT‐D induced‐IRI. All of these changes were returned to sham levels after EGCG treatment prior to reperfusion (group III). Conclusion EGCG, as a free radical scavenger, seems to have a protective role against oxidative stress in testicular IRI.