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Effect of quercetin‐3‐glucoside on gene expression in human colon cancer cells
Author(s) -
You Hyun Ju,
Ahn Hyung Jin,
Ji Geun Eog
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb455
Subject(s) - kegg , endoplasmic reticulum , microbiology and biotechnology , gene expression , biology , gene , biochemistry , transcriptome
To investigate the mechanisms regarding anti‐proliferative effect of quercetin‐3‐glucoside (Q3G) on colon cancer cells, gene‐expression analysis of HT‐29 cells treated with Q3G was performed by microarray. Pathway analysis of differentially expressed genes by using R program, PANTHER, DAVID and GSEA bioinformatics tools showed changes in steroid biosynthesis (KEGG) and endoplasmic reticulum membrane (GOTERM) pathways after 12 hr treatment with 25 μM of Q3G. Q3G also induced distinctive morphological changes of cells and the effects of extracellular matrix‐associated integrin and related pathways on morphology were investigated by quantitative reverse transcriptase PCR. The changes in the expression of regulatory molecules such as p21 CIP1 , cyclin G2, ATF3, TRIB3, DDIT4, AKAP13, BHLHB2, RAP1GA1, and KLF6 were supposed to be associated with Q3G‐induced cell cycle arrest and apoptosis. This work was supported by the Technology Development Program (No. 110124‐2‐2‐SB010) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries and the Next‐Generation BioGreen 21 Program (No. PJ008005), Rural Development Administration, Republic of Korea.