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Immuno‐stimulating Activities of Polysaccharides from Cheonggukjang
Author(s) -
HONG HEE-DO,
CHO CHANG-WON,
HAN CHUN-JI,
RHEE YOUNG KYOUNG,
NAM SO-HYUN,
KIM YOUNG-CHAN
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb332
Subject(s) - polysaccharide , splenocyte , ex vivo , chemistry , in vivo , uronic acid , in vitro , spleen , ethanol precipitation , pharmacology , traditional medicine , immunology , biochemistry , medicine , biology , microbiology and biotechnology
In this work, we examined in vitro , ex vivo and in vivo immuno‐stimulating activities of polysaccharides from Cheonggukjang . The crude polysaccharide (CHW‐0) was isolated from Cheonggukjang by hot water extraction, ethanol precipitation, and ultrafiltration. The major constituents in CHW‐0 were neutral sugar (51.4%), and the uronic acid (16.3%). The result of in vitro assay, CHW‐0 showed an anti‐complementary activity (ITCH 50 at 1,000 μg/ml: 30.3%). The immuno‐stimulating effects of CHW‐0 were also analysed ex vivo by means of nitric oxide (NO) and cytokines in supernatants of mouse peritoneal macrophage. The mouse peritoneal macrophage stimulated with CHW‐0 produced NO and various cytokines such as IL‐6 and IL‐12 in a dose‐dependent manner. We also investigated the immuno‐stimulating actions of CHW‐0 in 30 male Chinese Kun Ming (KM) mice. The mice were divided into three groups, and treated by oral administration with CHW‐0 into two dosages (100 mg/kg and 200 mg/kg body weight) for 4 weeks. The results showed that there were no significant differences in body weight and thymus index, but spleen index was increased. In the splenocytes proliferation test, the T and B lymphocyte proliferation abilities of CHW‐0 were significantly increased than that of control group. Overall, Cheonggukjang crude polysaccharide may have immuno‐stimulating activities beneficial to human health.