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Impact of weight loss through calorie restriction on iron status, inflammation and hepcidin expression in circulation and adipose tissue of obese individuals
Author(s) -
Dao Maria Carlota,
Ruth Megan R,
Karastergiou Kalypso,
Apovian Caroline M,
Fried Susan K,
Meydani Simin Nikbin
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb320
Subject(s) - hepcidin , iron status , inflammation , adipose tissue , medicine , calorie restriction , obesity , endocrinology , cd68 , weight loss , systemic inflammation , iron deficiency , immunohistochemistry , anemia
In obesity, hepcidin (Hep) is upregulated systemically and in adipose tissue (AT), which may lead to iron status impairment. It is not known how weight loss (WL) through calorie restriction (CR) affects Hep and iron status in obese adults. We assessed the effect of WL through CR in 24 obese adults, hypothesizing that systemic Hep and inflammation (Infl) would decrease with WL while iron status would be enhanced, and that WL would modulate Infl and Hep in AT. Serum Hep, CRP and iron status were assessed before and after WL. Subcutaneous AT biopsies were collected at both time points. Hep and macrophage marker expression was assessed by RT‐PCR. WL correlated with decreased serum Hep (r=−0.7, p=0.01) in subjects with 5–10% body WL (n=14). There was a trend for decreased serum CRP and increased iron status. Expression of CD68, a macrophage marker, was highly correlated with Hep expression before (r=0.7, p<0.001) and after (r=0.8, p<0.0001) WL. This data shows a significant effect of WL on Hep, and suggests a tight association between AT macrophages and Hep. Further research is needed to determine the role of inflammatory dysregulation of AT in obesity‐related iron status impairment. Support: Atkins Foundation, Boston University Clinical and Translational Science Institute M01 RR000533 and U54 RR025771, USDA HNRCA Pilot grant, NIH T32 and P30DK046200.