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Stromovascular Cell Differentiation in Collagen Matrix Induced by High Fat Medium
Author(s) -
Ettinger Susan,
White Joshua,
Brane Luke,
Kunza Brittany,
Agaronov Alan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb313
Subject(s) - extracellular matrix , adipocyte , adipose tissue , matrix (chemical analysis) , microbiology and biotechnology , cellular differentiation , adipogenesis , collagenase , chemistry , lipid droplet , endocrinology , biology , anatomy , medicine , biochemistry , chromatography , gene , enzyme
Stromovascular Cells (SVCs) derived from adipose tissue have potential to differentiate into cells of mesodermal origin such as adipocytes, cartilage, bone, and skeletal muscle. To study the influences of extracellular matrix and substrate on SVC differentiation, we cultured SVCs in a three‐dimensional (3D) collagen‐I matrix or on a two dimensional tissue culture surface (2D) with lipid supplemented and low lipid medium. Samples taken from three fat depots (inguinal, epididymal, and retroperitoneal) of adult male rats were treated with collagenase and the SVCs plated on either 3D or 2D tissue culture plates. Cells were incubated in 10% marine‐derived lipid supplemented media (HL) or low‐lipid control media (LL) for 4 weeks. SVCs in LL media proliferated more rapidly in 3D than 2D matrix; no evidence of differentiation was observed. Differentiation was only seen in SVCs cultured in HL medium on 3D matrix. (see figure). SVC differentiation to mature adipocytes has previously been shown to require dexamethasone, insulin and other growth stimulators. In conclusion, in our 3D model, SVCs grown in 10% lipid supplemented medium underwent robust adipocyte differentiation without growth stimulators. We propose that the 3D model, by replicating matrix binding and stresses in the in vivo environment, may allow a more physiologically relevant examination of factors that regulate SVC proliferation and differentiation.