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Fermented mixture of Rhodiola rosea and Korean red ginseng protects differentiated C2C12 cells from hydrogen peroxideinduced oxidative stress associated with antioxidant enzyme responses
Author(s) -
Yoon Bo ra,
Lee Young Jun,
Hong Hee Do,
Sung Su Kyung,
Lee Ok Hwan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb292
Subject(s) - rhodiola rosea , ginseng , oxidative stress , antioxidant , superoxide dismutase , rhodiola , chemistry , myogenesis , glutathione peroxidase , biochemistry , reactive oxygen species , c2c12 , medicine , salidroside , chromatography , alternative medicine , pathology , in vitro
Rhodiola species have anti‐fatigue, anti‐stress, antioxidant and immune enhancing effects. Skeletal muscle generates reactive oxygen species, which contributes to muscle fatigue. This study investigated the inhibitory effects of the fermented mixture of Rhodiola rosea and red ginseng on H 2 O 2 induced oxidative damage in C2C12 myotubes by analyzing morphological changes, cell viability, and gene expression level of antioxidant enzyme responses. C2C12 myoblasts were treated with fermented mixture for up to 5 days. Our data showed that the fermented mixture of Rhodiola rosea and red ginseng significantly protected oxidative damage during the myogenesis compared to H 2 O 2 treated C2C12 cells in a dose dependent manner. The fermented mixture of Rhodiola rosea and red ginseng also markedly enhanced mRNA expression of Cu/Zn‐superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as MyoD, a muscle‐specific transcription factor. Our results suggest that the inhibitory activity of the fermented mixture of Rhodiola rosea and red ginseng on oxidative damage may involve coordinated changes of cellular antioxidant enzymes in C2C12 cells.