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Simplified Methods for the Study of Cell Cycle and Apoptotic Impacts of Anti‐Cancer Compounds
Author(s) -
Tran Kimvan,
Tyagarajan Kamala
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb248
Subject(s) - apoptosis , cell cycle , nocodazole , gambogic acid , cell cycle checkpoint , etoposide , annexin , chemistry , cancer cell , mode of action , jurkat cells , cell , cell growth , cancer research , microbiology and biotechnology , computational biology , cancer , biology , biochemistry , immunology , t cell , genetics , chemotherapy , immune system , cytoskeleton
Several anticancer compounds have shown to have impacts on both cell cycle and cell health with differential impacts in the timing and sequence of each of these processes. Drug development studies therefore need simple accessible simple and easy methods to study impacts on both cell cycle and multiple apoptotic markers to understand the complete dimension of action of compounds. In this study we present data from rapid simplified methods utilized to study the impact of multiple anti‐cancer and anti‐tumor compounds on cell cycle arrest and apoptosis utilizing no‐wash assays. Studies were performed with Jurkat cells with multiple anti‐cancer compounds such as gambogic acid, etoposide, celastrol, and nocodazole for differential treatment conditions and the impact on cell cycle and impact on apoptosis by annexin V binding and caspase activation was evaluated. Our results indicate that the impacts and kinetics of cell cycle versus apoptosis markers can vary with different compound treatments. Screening for both cell cycle and apoptosis impacts are thus critical in understanding the full dimensions of mode of action of compounds and can be valuable in determining optimal conditions of use.