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Oxidized lipids and lysophosphatidylcholine (LPC) induce the chemotaxis and intracellular calcium influx in human natural killer (NK) cells
Author(s) -
Rolin Johannes,
Al-Jaderi Zaidoon,
Maghazachi Azzam A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb219
Subject(s) - chemotaxis , chemistry , lysophosphatidylcholine , intracellular , microbiology and biotechnology , pertussis toxin , receptor , biochemistry , biology , g protein , phosphatidylcholine , phospholipid , membrane
We previously reported that human NK cells express G2A and they respond to LPC. Here we report that oxidized lipids such as 9‐ R‐HODE, 9‐S‐HODE and 13‐R‐HODE, as well as LPC induced the in vitro chemotaxis of human NK cells, although with variable efficacies. The chemotactic effects of these lipids were inhibited by prior treatment of NK cells with pertussis toxin. Further, anti‐G2A inhibited the chemotactic effects of all four lipids examined. 9‐S‐HODE, 9‐R‐HODE and LPC optimally induced the influx of intracellular Ca 2+ in NK cells. Addition of 9‐S‐HODE prior to the addition of LPC inhibited more than 50% of the effect of LPC, whereas addition of LPC prior to the addition of 9‐S‐HODE completely inhibited the effect of the latter lipid. However, there was a complete reciprocal desensitization among 9‐R‐HODE and LPC on the influx of intracellular Ca 2+ . Further analysis showed that 1 μM concentration of all four lipids inhibited NK cell lysis of tumor target cells. These lipids did not affect the expression of chemokine receptors on NK cells. In contrast, we observed increased expression of CCR7, CCR9, CXCR1 and CXCR4 (9S‐HODE also increased expression of CCR6), but not CCR1, CCR2, CCR3, CCR4, CCR5, CXCR3, CXCR5 or CXCR6 after incubation of monocytes with these lipids. This was corroborated with the ability of lipid‐treated monocytes to respond chemotactically towards MIP‐3β/CCL21 and IL‐8/CXCL8, albeit with variable efficacies. In conclusion, we provide evidence showing that oxidized lipids and LPC exert important functions for cells of innate immune system.