Premium
Expression of PPARα, β, and γ in the Hartley guinea pig model of primary osteoarthritis
Author(s) -
El Mansouri Fatima Ezzahra,
Nebbaki Salwa Sarah,
Zayed Nadia,
Benderdour Mohamed,
Martel-Pelletier Johanne,
Pelletier Jean-Pierre,
Fahmi Hassan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.lb130
Subject(s) - osteoarthritis , peroxisome proliferator activated receptor , cartilage , medicine , receptor , endocrinology , immunohistochemistry , pathogenesis , guinea pig , nuclear receptor , peroxisome , pathology , biology , anatomy , transcription factor , gene , biochemistry , alternative medicine
Background Peroxisome proliferator‐activated receptors (PPARs) are members of the nuclear receptor superfamily (PPARα, PPARβ/δ and PPARγ). Little is known about the pattern of PPAR expression during the progression of osteoarthritis (OA). Objective To investigate the expression of PPARα, β, and γ in cartilage over the course of OA in the spontaneous Hartley guinea pig model. Methods Hartley guinea pigs were sacrificed at 2, 4, 8, and 12 month‐old of age. Cartilage was obtained from the central portion of the medial tibial plateau. Cartilage degradation was evaluated histologically using the Osteoarthritis Research Society International (OARSI) guidelines. The expression of PPARα, β and γ was analyzed by immunohistochemistry. Results PPARα, β and γ, were detected in medial tibial plateaus from control animals. There was no significant changes in the levels of PPARα and PPARβ over the course of OA. In contrast, PPARγ expression decreased during the progression of OA. Correlation analysis revealed a negative correlation between PPARγ levels and histological score of OA. Conclusion Expression of PPARγ in cartilage decreased during OA. These data suggest that loss of PPARγ expression in cartilage may contribute to the pathogenesis of OA.