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Time‐dependent changes in long range sphingolipid organization revealed by high‐resolution secondary ion mass spectrometry
Author(s) -
Kraft Mary L,
Klitzing Haley A.,
Lou Kaiyan,
Zimmerberg Joshua,
Weber Peter K.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.987.1
Subject(s) - sphingolipid , membrane , chemistry , resolution (logic) , mass spectrometry , biophysics , lipidomics , secondary ion mass spectrometry , cell membrane , biochemistry , biology , chromatography , artificial intelligence , computer science
To coordinate cell signaling and other membrane‐mediated cellular functions, the cellular plasma membrane is organized into domains of differing protein and lipid composition. Though the distributions of specific membrane proteins can be imaged with many techniques, the precise spatial organization of sphingolipids within the plasma membrane is not well‐established. Here we have combined metabolic isotope incorporation with high‐resolution secondary ion mass spectrometry (SIMS) to chemically image the distributions of sphingolipids within the plasma membranes of intact fibroblast cells with at least 100 nm lateral resolution. Using this approach, we characterize how the sphingolipid distribution changes as a function of metabolic pulse‐labeling time. We show that changes in the short range (<1 micron) sphingolipid organization are not detectable, but the non‐random sphingolipid organization occurring on longer length scales (>5 microns) varies with the duration of selective isotope incorporation.