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Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/Bs in Jurkat cells
Author(s) -
Yeh Yuan-ming,
Huang Pei-Rong,
Wu Chih-Ching,
Wang Tzu-Chien Vant
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.979.1
Subject(s) - jurkat cells , heterogeneous nuclear ribonucleoprotein , microbiology and biotechnology , biology , heterogeneous ribonucleoprotein particle , gene , ribonucleoprotein , rna interference , proteome , in vitro , chemistry , genetics , rna , t cell , immune system
The hnRNP A/Bs (A1, A2/B1, and A3) are the most abundant proteins in the nuclear extracts that bind specifically to G‐strand telomeric DNA. While the telomeric DNA‐binding activities of hnRNP A/Bs in vitro have been characterized in detail, the biological functions of hnRNP A/Bs in vivo remains poorly understood. In this study, we have employed RNAi knock‐down and proteomic approach to investigate the biological functions of hnRNP A/Bs. Knock‐down of hnRNP A2, but not A1 or A3, produced a profound effect on cellular proliferation in Jurkat cells. A slight increase of apoptotic cells was observed in the A2‐knock‐down cells, but not in the A1 or A3 knock‐down cells. Analysis of the proteomes in the cells singly knock‐down for hnRNP A1, A2 or A3 has identified a total of 1604 quantifiable proteins. Among the down‐regulated proteins, one was commonly regulated by A1 and A3, three were commonly regulated by A1 and A2, and three were commonly regulated by A2 and A3. A total of 19 proteins were specifically altered only in the cells knock‐down for A2. Studies are underway to investigate biological functions of A2‐regulated genes. This study was supported by research grant CMRPD1A0421.