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Distribution and localization of Rho GTPases in rat brain
Author(s) -
Martínez-Alcantar Lorena,
Meléndez-Herrera Esperanza,
Clemente-Guerrero Mónica,
Saavedra-Molina Alfredo,
Manzo-Avalos Salvador
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.973.2
Subject(s) - rhoa , rhob , gtpase , microbiology and biotechnology , cytoskeleton , biology , rac1 , neurite , rhoc , chemistry , cell , signal transduction , biochemistry , in vitro
Rho GTPases proteins are essential for cytoskeletal reorganization. Several studies have implicated members of the Rho GTPase family in the neuronal polarization and the formation of axons and dendrites. Only few studies have investigated the cellular distribution of RhoA, RhoB and Rac1. Therefore the aim of this study was to investigate the cellular distribution of these proteins using immunohistochemical technique. Rac1, RhoA and RhoB proteins show a differential distribution and the pattern is different in rat brain. RhoA was localized in the soma and dendrites of neurons in most brain regions, hypothalamus. In brainstem nucleus RhoA showed increased expression. RhoB showed a more restricted distribution, and was located in the brain stem and in the plasma membrane in the pyramidal cell layer of the cortex. Rac1 was localized in neuronal cell bodies and distributed in all brain regions. The differential localization of the Rho GTPases in rat brain may reflect different roles of these proteins within these structures.