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In vivo microdialysis sampling of adipokines from rat mammary fat pad. Comparison of dialysates to whole tissue concentrations
Author(s) -
Bajpai Geetika,
Stenken Julie A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.971.4
Subject(s) - microdialysis , adipokine , adipose tissue , in vivo , chemistry , medicine , endocrinology , biology , obesity , leptin , microbiology and biotechnology
Objective Microdialysis sampling was used to collect adipokines, CCL2 (MCP‐1) and IL‐6, from mammary fat pads of female rats (Figure 1). A critical issue with microdialysis sampling is the calibration and determining actual tissue concentrations. Tissue concentrations of the adipokines were obtained and compared with dialysates. Methods CMA 20 polyethersulfone, 100 kDa MWCO, probes were implanted in the right abdominal mammary fat pad of female Sprague‐Dawley rats (220–250g). Tissue around the probe was harvested for protein extraction. CCL2 and IL‐6 were quantified using Luminex and ELISA kits. Results and discussion Concentrations of IL‐6 and CCL2 increased after probe implantation (Table 1). Interestingly, IL‐6 levels were not different, but CCL2 levels were between the implanted vs. control tissue. This may suggest that IL‐6 levels are not influenced by the probe insertion trauma. Conclusions Adipokines can be collected from mammary fat pad using microdialysis sampling. Up to ~50 fold differences exist between tissue and dialysate levels. Funding : COBRE 1P30RR031154‐01 and Arkansas Biosciences Institute.