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Yeast Aging Proteome Unveiled a Novel Aging Regulation Pathway Mediated by the Chromatin Remodeling Complex ISW2
Author(s) -
Dang Weiwei,
Jansen Pascal,
Dorsey Jean,
Cao Kajia,
Perry Rocco,
Kaeberlein Matt,
Kennedy Brian K,
Vermeulen Michiel,
Berger Shelley
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.965.2
Subject(s) - calorie restriction , biology , chromatin , proteome , chromatin remodeling , stable isotope labeling by amino acids in cell culture , yeast , proteomics , genetics , gene , endocrinology
Calorie restriction is known as the most robust intervention to extend lifespan for eukaryotes, from yeast to primates. Although numerous conserved aging regulators and pathways involved in calorie restriction have been identified over the years, the overall proteomic changes during aging or under calorie restriction conditions remain largely unexplored. We compared proteomes between young and replicatively aged yeast cells under normal and calorie restricted conditions using the quantitative proteomics method, SILAC. Analysis of proteomic changes under these conditions unveiled distinct signatures in the aging proteome and the similarity between the aging and calorie restriction proteomes. These discoveries provided clues to a novel aging regulation pathway mediated by the chromatin remodeling complex ISW2. Disruption of ISW2 mimics the calorie restriction conditions by elevating cellular response to stress and extends replicative lifespan. This study presents the first evidence that chromatin remodeling plays an important role during aging and that yeast cells exploit the benefits of calorie restriction in the later stages of their lives.