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Property of the antioxidant activity using xanthine oxidase reaction
Author(s) -
Masuoka Noriyoshi,
Maeta Ayami,
Kamimura Wakana,
Kubo Isao
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.963.2
Subject(s) - chemistry , xanthine oxidase , antioxidant , xanthine , hydrogen peroxide , superoxide , uric acid , reactive oxygen species , reagent , oxidase test , catalysis , enzyme , biochemistry , organic chemistry
Inhibitory activity of the superoxide anion (O 2 − ) generation catalyzed by xanthine oxidase (EC 1.1.3.22 ) with natural products was used to estimate the antioxidant activity. To understand properties about the activity, we examined antioxidant activity of anacardic acids, alkyl gallates and flavonoids. Xanthine oxidase consisted of several oxid‐reduction forms in the molecule. The oxidized forms catalyzed O 2 − generation from oxygen but the reduced forms catalyzed only hydrogen peroxide formation. We deduced that antioxidants affected three reactions, (A), (B) and (C) to indicate antioxidant activity. In reaction (A), antioxidants function inhibitors of xanthine oxidase reaction, and both uric acid formation and O 2 − generation were suppresses. In the reaction (B), antioxidants function as reducing reagents of xanthine oxidase, and the reduced enzyme cannot generate O 2 − . In the reaction(C), antioxidants function as scavengers of O 2 − generated by xanthine oxidase. We also indicate that effects of antioxidants on reaction (A) and (B) are stronger than those on reaction (C).