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Identification of Potent inhibitors of Bacillus anthracis FtsZ: A target for antimicrobial agents
Author(s) -
Yoon Moon-Young,
Park Hae-Chul
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.962.3
Subject(s) - ftsz , recombinant dna , tubulin , escherichia coli , bacillus anthracis , biology , antimicrobial , chemistry , microbiology and biotechnology , polymerization , biochemistry , bacteria , microtubule , gene , genetics , organic chemistry , polymer
FtsZ plays an essential role in cell division and identified as antimicrobial target. The expression plasmid carrying Bantx‐FtsZ gene was expressed in Escherichia coli BL21 (DE3) cells and the expressed recombinant Bantx‐FtsZ was purified under the soluble conditions using Ni2+ charged sepharose. The purified recombinant Bantx‐FtsZ yield was estimated to be 3.5 mg/L culture. Guanosine 5‐triphosphate (GTP) dependent polymerizations of recombinant Bantx‐FtsZ were confirmed with both turbidity at A350 and Transmission Electronic Microscope (TEM). The polymerized Bantx‐FtsZ structure was confirmed with TEM that was filamentous shaped. The minimum‐required and saturation concentrations for polymerization of Bantx‐FtsZ were found to be 0.012 and 0.2 mg/mL, respectively. Berberine, a known E. coli FtsZ polymerization inhibitor, inhibited the Bantx‐FtsZ polymerization with an IC50 value of 60 ± 4.4 μM. In addition, our efforts aimed to identify potent inhibitors of Bantx‐FtsZ polymerization from a naturally extracted chemical library yielded 3 chemicals with IC50 values ranges from 10 to 100 μM. Understanding the biochemical basis and growth inhibition of B.anthracis of these inhibitors in future is essential for the identification of potential new anti‐anthracis drugs.