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Dissecting the interactions between Human Argonaute proteins and RISC components
Author(s) -
Chen Yen-Chun,
Huang Jin-Wen,
Chu Chia-Ying
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.952.3
Subject(s) - argonaute , rna induced silencing complex , gene silencing , microrna , microbiology and biotechnology , rna interference , biology , rna , gene , genetics
MicroRNAs (miRNAs) are a group of non‐coding RNAs, with ~20–24 nt in length, that regulate gene expression at post‐transcriptional level. In human cells, miRNAs are loaded to Argonaute proteins (AGOs), which interact with other proteins to form RNA‐induced silencing complex (RISC). miRNAs then guide RISC to recognize their target mRNAs. These miRNA‐RNA‐protein complexes will trans‐locate to a specific cytoplasmic compartment called RNA processing body (P‐body), for the mRNA storage or degradation. It has been revealed that RCK/p54 (DDX6) is the key protein for P‐body formation and it also interacts with AGO2. Nevertheless, how RCK/p54 interacts with AGO2 to facilitate the miRNA‐mediate gene regulation in P‐body remains unclear. Here we report the domains and residues of AGO2 required for interacting with RCK/p54. Our preliminary data shows that the localization of AGO2 at various cytoplasmic RNA granules and the interaction with RCK/p54 are determined by different domains of AGO2, suggesting that miRNA‐mediated translational repression is unrelated to the miRISC localization at P‐body. Our continuing analysis of AGO2 deletion mutants interacting with other RISC components will have important implications for the miRNA‐mediated gene silencing.