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RELATIONSHIP OF GENE EXPRESSION AND SEX HORMONE RECEPTORS IN PRIMARY BREAST CARCINOMA AND METASTASES FROM THE SAME PATIENT
Author(s) -
Andres Sarah Alane,
Wittliff James L.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.928.5
Subject(s) - estrogen receptor , breast cancer , cancer research , metastasis , biology , breast carcinoma , primary tumor , lymph node , tissue microarray , cancer , gene expression , pathology , oncology , gene , medicine , genetics
Using microarray results and published reports, 32 genes were selected whose expression appears related to clinical outcome of breast cancer. Candidate gene expression and estrogen (ER) and progestin receptor (PR), determined by ligand binding or EIA, were evaluated to ascertain their relationship during lymph node invasion. RNA was isolated from tissue sections of de‐identified frozen carcinoma biopsies. cDNA was prepared with Superscript RT III reagents (Invitrogen) and qPCR reactions were performed using Power Sybr Green PCR Master Mix (Applied Biosystems). Although levels of ER and PR protein varied between the primary and the metastasis, no primary lesion that was ER− or PR−exhibited a positive ER/PR result in the metastasis. Only 7 patients with either ER+ or PR+ primaries exhibited loss of receptor expression in the metastasis. ESR1 and PGR gene expression correlated highly with protein levels of ER and PR. Expression of 6 of 32 genes (TBC1D9, TRIM29, SCUBE2, IL6ST, SLC39A6, LRBA) was discordant in at least half of the primary lesion‐lymph node pairs, while expression of 6 genes (TRIM29, SCUBE2, SLC39A6, TCEAL1, PFKP, XBP1) was discordant in at least half of the primary‐distant metastasis pairs. These results address the molecular basis for progression of a primary lesion to a metastatic phenotype. Supported in part by grants from Phi Beta Psi Charity Trust and a CTSP Grant from the Commonwealth of Kentucky.