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Multilayered, Multi‐Component Anti‐infective Nanocoatings for Biomedicine
Author(s) -
Mills David,
Elfer Kate,
McNamara Kaitlin,
Manuel Morganne,
Lvov Yuri
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.917.1
Subject(s) - gentamicin , materials science , antibiotics , nanotechnology , microbiology and biotechnology , biology
Nebulization was used to build multilayered polyelectrolyte nanocoatings (PMNs) onto poly‐e‐caprolactone (PCL) scaffolds. Nebulization creates surface nanocoatings through the conversion of polyelectrolyte solutions that can be sprayed directly onto a surface. This technique permits assembly of multilayer scaffolds with tunable architectures with the potential for diverse biomedical applications. PMNs were created and tested as anti‐infective (gentamicin‐based) nanocoatings. PLL and PGA were used to synthesize a ten‐bilayer structure. Gentamicin was embedded within each PMN using two different antibiotic gradients: deep to superficial and superficial to deep. PCL/PMNs/Gentamicin scaffolds were then placed onto bacteria‐laded agar plates over a 14‐day period. Image analysis was used to assess bacterial response to scaffolds. Controls consisted of PCL/PMNs scaffolds without gentamicin. Results showed that both antibiotic architectures proved effective in killing bacteria and controlling bacterial growth over the experimental period with the deep to superficial structure having the most profound effect. A biodegradable bandage consisting of the multi‐component (growth factor/antibiotic) nanocoatings is currently being tested for its anti‐infective/ regenerative properties. Louisiana Tech's NSF REU program and the Governor's Biotechnology Initiative provided funding support.