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Design of bioactive smart nanofilms with bFGF and PDGF‐BB for wound repair
Author(s) -
Challagundla Sukeerth,
Mills David K.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.916.9
Subject(s) - wound healing , dermis , growth factor , skin repair , platelet derived growth factor receptor , hyaluronic acid , platelet rich plasma , biomedical engineering , materials science , chemistry , medicine , surgery , pathology , platelet , immunology , anatomy , biochemistry , receptor
Wound healing has become a prominent area of research in regenerative medicine. Although many wound healing and wound dressing techniques have been developed, there remains a great demand for an enhanced treatment modality. Fibroblast Growth Factor (bFGF) and Platelet Derived Growth Factor –BB (PDGF– BB) were incorporated into a polyelectrolyte multilayer nanocoating (PMNs) and applied to a wounded skin equivalent (Epiderm™). The nanocoating consisted of a ten‐layer Poly‐L‐Lysine (PLL) and Poly‐Glutamic‐Acid (PGA) nanocoating with growth factors embedded in two different modalities, a deep layer and double layer. Tissues treated with the designed PMNs enhanced re‐epithelialization, collagen synthesis and the rate of wound healing significantly. The degree of tissue response varied with each nanocoating. The double layer modality showed improved healing compared to the single modalities with an increase in cell number at the wound site with greater proliferation in the epidermis compared to the dermis. Picrosirius Red staining results showed an increase in the level of collagen deposition in the dermis. Controls, with a ten‐layer nanocoating, and no growth factors, showed marginal wound repair. These results suggest that bioactive nanocoatings may have potential as a treatment modality for cutaneous and chronic wounds. Funding provided by the State of Louisiana Governor's Biotechnology Initiative.