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Mesenchymal stromal cells (MSC) and platelet rich plasma (PRP) on a collagen matrix improve the tensile strength of a repaired incision in a rat model
Author(s) -
Fagan Diana,
Heffner Jeremy,
Holmes Jon,
Ferrari Jon,
Krontiris-Litowitz Johanna,
Perko John,
Dorion Heath,
Marie Hazel
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.915.4
Subject(s) - platelet rich plasma , medicine , ultimate tensile strength , wound healing , mesenchymal stem cell , surgery , hernia repair , hernia , matrix (chemical analysis) , chemistry , platelet , pathology , materials science , metallurgy , chromatography
The goal of this project is to prevent hernia formation by improving wound healing in surgically repaired tissue. Midline abdominal incisions were performed on Lewis rats. In Group 1 (n=14) the incision was repaired with no further additions. Group 2 had PRP on a collagen matrix (CollaTape™, Zimmer Dental) applied to the repaired incision. Group 3 received the same treatment as Group 2 with the addition of 10 6 MSC. The biomechanical properties of the facial defects, including the control samples, were determined using a standard force‐extension tensiometric analysis after 4 weeks (n=7) or 8 weeks (n=7). Tissues from Group 1 had an average tensile strength of 204 kPa at four weeks and 421.83 kPa at eight weeks; Group 2 at four weeks was 409.58 kPa and 582.18 kPa at eight weeks; Group 3 at four weeks was 817.15 kPa and at eight weeks was 917.17 kPa. Marked improvement in healing was seen with PRP, which was further improved by the addition of MSC. Supported by St. Elizabeth Health Center, Youngstown State University and LifeCell Hernia Resident/Fellow Research Grant.