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The use of mesenchymal stromal cells improves wound repair in rat abdominal incisions
Author(s) -
Minteer Tanya Elise,
Heffner Jeremy,
Holmes Jonathan,
Ferrari Jonathan,
Krontiris-Litowitz Johanna,
Marie Hazel,
Perko John,
Dorion Heath,
Fagan Diana
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.915.3
Subject(s) - medicine , mesenchymal stem cell , surgery , fascia , trichrome stain , abdominal wall , abdominal hernia , platelet rich plasma , wound healing , stromal cell , abdominal surgery , platelet , pathology , immunohistochemistry
More than 200,000 abdominal hernias are repaired each year. Although new surgical techniques and technologies have attempted to combat the occurrence of hernias, patients continue to suffer from hernias following abdominal surgeries. Regenerative therapy, including the use of bone marrow derived‐mesenchymal stromal cells (MSCs), offers a new avenue to reducing the problem of hernias seen in post‐operative patients. Midline laparotomies were performed on three groups of Lewis rats. Group 1 rats were designated as the control group and were repaired without additives. CollaTape containing platelet‐rich plasma (PRP) was used to repair the abdominal incisions of group 2 rats. The incisions of group 3 rats were repaired similarly to group 2 but with the addition of MSCs. At 4 and 8 weeks postoperative, abdominal fascia was excised (n=7 per group). Collagen deposition and organization (as determined by a Trichrome stain) was rated by blinded observers. Group 3 showed significantly increased neovascularization and collagen deposition compared to the abdominal fascia of groups 1 and 2. The results of this study suggest that the use of MSCs in abdominal repairs could serve as a beneficial tool to improve wound healing. Grant Funding Source : LifeCell Hernia Resident/Fellow Research Grant