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Effects of laminar shear stress on expression of MT1‐MMP in rat bone marrow‐derived mesenchymal stem cells
Author(s) -
Qin Wang Han
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.913.8
Subject(s) - wortmannin , shear stress , mesenchymal stem cell , laminar flow , chemistry , bone marrow , pi3k/akt/mtor pathway , protein kinase b , microbiology and biotechnology , biology , materials science , signal transduction , immunology , physics , composite material , mechanics
Objective To investigate the effect of laminar shear stress on the expression of membrane type‐1 matrix metalloproteinase (MT1‐MMP) in rat bone marrow‐derived mesenchymal stem cells (rMSCs). Methods rMSCs were isolated from SD rat lower extremities and loaded by laminar shear stress with the parallel‐plate flow chamber system, and then the expression of MT1‐MMP mRNA of rMSCs was examined by real‐time PCR. Results Shear stress with different magnitude of 5, 15, 30 dynes/cm2 all induced MT1‐MMP mRNA expression, especially high shear stress, 30 dynes/cm2, which was time‐dependent. The expression of MT1‐MMP mRNA was inhibited by SB202190, an inhibitor of p38, but enhanced by wortmannin, an inhibitor of PI3K/Akt. Conclusion Shear stress can activate the expression of MT1‐MMP. The amount of MT1‐MMP expression is closely related to stimulating time and the strengths of shear stress, and p38MAPK and PI3K/Akt signal pathway may play a critical role during the process.