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Cell fusion phenomena was detected after xenogenic intra‐uterus transplantation of Ds‐red porcine amniotic fluid derived stem cells into EGFP bearing mice
Author(s) -
peng shao-yu,
Chou Chih-Jen,
Wu Shinn-Chih
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.913.7
Subject(s) - amniotic fluid , stem cell , amniotic stem cells , andrology , transplantation , fetus , endoderm , uterus , amnion , biology , green fluorescent protein , ectoderm , microbiology and biotechnology , medicine , embryo , embryonic stem cell , embryogenesis , adult stem cell , pregnancy , endocrinology , genetics , gene
Amniotic fluid derived stem cells (AFSCs) are derived from amniotic fluid of developing fetus and can be given rise to three germ layers ‐ endoderm, mesoderm and ectoderm. Intra‐uterus transplantation (IUT) is an approach to cure the inherited genetic defect for the fetus during the gestation period of the pregnant female. Certain diseases such as osteogenesis imperfect, muscular destrophy, ect. were successfully treated with stem cells to ameliorate the syndrome. The purpose of this study was to investigate the bio‐distribution and engraftment of porcine amniotic fluid derived stem cell (pAFSCs) after IUT and further determine the fate of the donor cells through Ds‐red harboring pAFSCs inject into EGFP‐harboring fetus of the pregnant mice. The 12.5 days pregnant mice were performed the IUT surgery by pAFSCs injection. One month after birth, the mice were sacrificed. Several portions from different organs were sampled for histological examination. Experiment results showed that the migration of Ds‐red pAFSCs were expressed most significantly in the intestines. Furthermore, the EGFP signal and RFP signal were co‐expressed in the detected cells. It is concluded that the exogenous implantation of pAFSC was fused with recipient cells instead of differentiation or maintain the undifferentiated status retaining in the tissue. Grant Funding Source : National Taiwan University