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Mobilization of very small embryonic‐like stem cells by G‐CSF in MACO mice
Author(s) -
Zheng Min,
Wang Jian
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.913.2
Subject(s) - medicine , bone marrow , stromal cell , embryonic stem cell , stem cell , ischemia , pathology , neural stem cell , chemistry , biology , microbiology and biotechnology , biochemistry , gene
Objective To investigate the effect of G‐CSF on neural restoration and on mobilization of very small embryonic‐like stem cells(VSEL‐SC) in bone marrow of focal cerebral ischemia mouse. Methods MCAO model was produced by filament occlusion method. 50¦Ìg/(kg.d) G‐CSF were given by subcutaneous injection for 5 days. Neurological scale was evaluated. The number of VSEL‐SC (CXCR4+Lin‐CD45‐) in peripheral blood was checked by fluorescence‐activated cell sorting analysis (FACS). Level of stromal derived factor‐1(SDF‐1) in plasma and infarct cerebral tissue were checked by ELISA. Expression of SDF‐1 in ischemia area was checked by immunohistochemical staining. Results G‐CSF treatment significantly decreased the neurological scale of MCAO model mice compared with controls. G‐CSF could mobilize VSEL‐SC in the bone marrow of focal cerebral ischemia mice into peripheral blood. The concentration of SDF‐1 in plasma and in cerebral tissue significantly increased in G‐CSF treated group. Expression of SDF‐1 in infarct region significantly increased in G‐CSF treated group. Conclusion The beneficial effect of G‐CSF may be related to the mobilization of VSEL‐SC and the promotion of SDF‐1 expression in the infarct tissue of MACO mice. This work was supported by National Scientific Foundation Committee of China ( 31100985)