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The expression pattern of echinoderm microtubule‐associated like proteins (EMLs) in zebrafish during caudal tail regeneration
Author(s) -
Dyer William C.,
Daggett Melissa A. F.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.909.6
Subject(s) - zebrafish , microtubule , biology , echinoderm , microbiology and biotechnology , cytoskeleton , mitosis , intermediate filament , regeneration (biology) , genetics , gene , cell , paleontology
Cells are comprised of three major cytoskeletal filaments; microtubules, microfilaments, and intermediate filaments. Proteins are known to associate with these filaments and ultimately help to carry out the filament's respective function. Microtubules in particular carry out essential functions such as chromosome segregation during cell division. A conserved family of proteins referred to as echinoderm microtubule‐associated like proteins (EMLs) has been identified in the genomes of a wide range of phyla. The original EML family member, the 77 kD echinoderm microtubule‐associated protein (EMAP), was characterized in sea urchin eggs and embryos where it is found on the mitotic spindle and co‐localizes with protein kinases important for cell‐cycle regulation. PCR analysis of cDNA isolated from zebrafish tissues indicates the presence of EML transcripts in zebrafish during all stages of development and in adult tissues including the caudal tail. The goal of this study is to investigate the expression pattern of the zebrafish EML homologs using a caudal tail regeneration system. Our results indicate that the expression of both zebrafish EML homologs goes down during caudal tail regeneration.