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Effect of Thyroid Hormone Exposure on Murine Calvarial Derived Pre‐Osteoblasts
Author(s) -
Cray James John,
Weinberg Seth M,
El-Salanty Mohammed
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.907.8
Subject(s) - calvaria , endocrinology , medicine , alkaline phosphatase , thyroid , craniosynostosis , neurocranium , hormone , biology , chemistry , in vitro , skull , anatomy , genetics , enzyme , biochemistry
Craniosynostosis (CS) is the premature fusion of one or more of the fibrous joints of the skull, which normally allow for the growth of the neurocranium. The incidence of CS is one in every 2000–3000 births. CS involves the overgrowth of bone at the osteogenic fronts of the developing cranial bones. The etiology of CS, whether genetic or environmental, is unknown for the majority of cases. Little is known about the role of environmental agents or gene‐environment interactions in modulating CS phenotypes. The clinical and epidemiological literature suggests that maternal hyperthyroidism is a risk factor associated with CS in offspring, however the mechanism of action is unclear. Here we provide a preliminary report of the effects of exogenous thyroid hormone exposure on cells derived from murine calvaria. Murine derived calvarial cells were assayed for proliferation and osteogenic differentiation after 24,72, 168 hours in culture with exogenous thryoxine at doses ranging from 10^−4 and 10^−16 mol. Exogenous exposure to thyroid hormone appears to stimulate cells to express higher levels of alkaline phosphatase (ALP) in a dose dependent manner. These differences reach 5 times the amount of ALP activity after exposure to the highest doses. There does not appear to be a pattern of difference for proliferation. Thus, it appears thyroid hormone may exert its effects on sutures by increasing the activity of osteoblasts. Grant Funding Source : None