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MicroRNA‐610: A novel regulator of gastric cancer migration
Author(s) -
Wang Jing,
Zhang Jingwei,
Wu Junzhu,
Luo Daji,
Su Ke,
Shi Wentao,
Tian Yihao,
Wei Lei
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.905.5
Subject(s) - microrna , cancer , metastasis , ectopic expression , cancer cell , transfection , western blot , regulator , cancer research , luciferase , suppressor , biology , downregulation and upregulation , cell culture , gene , biochemistry , genetics
The main cause of gastric cancer‐related death is more often due to local relapse and long‐distance metastasis. Our previous studies demonstrated that miR‐610, abundantly expressed in normal gastric tissue, showed a significant down‐regulation in SGC‐7901 gastric cancer cells. Conversely, a predicted miR‐610 target, VASP, was over‐expressed in gastric cancer tissue samples. We hypothesized a reciprocal interaction between miR‐610 and VASP. To show this, gastric cancer cells were transfected with a miR‐610 precursor vector to increase endogenous miR‐610 expression. Cell migration was measured by wound healing assay and tumor cell invasion was measured using transwell assay. The interaction between miR‐610 and VASP was ascertained using qPCR, Western Blot and luciferase reporter assays. We demonstrated that ectopic miR‐610 expression reduced the migratory and invasive phenotype of gastric cancer cells. A direct effect of miR‐610 on VASP expression levels was demonstrated at the protein levels via miR‐610 targeting the VASP mRNA 3′‐UTR. In summary, we show that miR‐610 functions as a tumour suppressor miRNA being downregulated in gastric cancer which results in overexpression of VASP. Delivery of miR‐610 into gastric cancer may represent as a novel therapeutic approach to limit gastric cancer metastasis.(The work was supported by the Fundamental Research Funds for the Central Universities No3081006.)